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hiscript ii reverse transcriptase  (Vazyme Biotech Co)


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    Structured Review

    Vazyme Biotech Co hiscript ii reverse transcriptase
    Hiscript Ii Reverse Transcriptase, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 97/100, based on 1477 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hiscript+reverse+transcriptase/HiScript+II+Reverse+Transcriptase/custom%40r201%4042495521
    Average 97 stars, based on 1477 article reviews
    hiscript ii reverse transcriptase - by Bioz Stars, 2026-09
    97/100 stars

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    Reverse Transcription:

    Article Title: Repurposing metformin as a dual-function agent to combat E. coli-induced mastitis: Mechanistic insights into biofilm dispersion and AMPK/SIRT1-mediated NF-κB inhibition.
    Article Snippet: The treated bacterial cells, bovine mammary cells, mice, or sheep mammary tissue were taken for total RNA extraction using the Bacteria RNA Extraction Kit or RNA-easy Isolation Reagent (Vazyme, China). .. 1 μg of RNA was reverse transcribed using HiScript Reverse Transcriptase (Vazyme, China). .. The RT-qPCR for synthesized cDNA was performed with SYBR AceQ qPCR SYBR Green Master Mix (Vazyme, China).

    Article Title: Repurposing metformin as a dual-function agent to combat E. coli -induced mastitis: Mechanistic insights into biofilm dispersion and AMPK/SIRT1-mediated NF-κB inhibition
    Article Snippet: The treated bacterial cells, bovine mammary cells, mice, or sheep mammary tissue were taken for total RNA extraction using the Bacteria RNA Extraction Kit or RNA-easy Isolation Reagent (Vazyme, China). .. 1 μg of RNA was reverse transcribed using HiScript Reverse Transcriptase (Vazyme, China). .. The RT-qPCR for synthesized cDNA was performed with SYBR AceQ qPCR SYBR Green Master Mix (Vazyme, China).

    Article Title: Combinatorial inhibition of IGF2BP3-regulated receptor tyrosine kinases offers a new therapeutic strategy for triple-negative breast cancer
    Article Snippet: RNA-protein complexes were immunoprecipitated using Protein A/G magnetic beads (MedChemExpress, New Jersey, USA) for 2 h at 4 °C. .. Following extensive washing with RIP wash buffer, bound RNAs were eluted, purified using the RNeasy Mini Kit (Qiagen, Hilden, Germany), reverse-transcribed into cDNA with HiScript ® Reverse Transcriptase (Vazyme, Nanjing, China), and quantified by qRT-PCR. .. For MeRIP, total RNA was extracted using TRIzolTM reagent (Takara, Kyoto, Japan).

    Article Title: miRNA-29a inhibits the proliferation of HUVECs by regulating the ITGB1/β-catenin/c-Myc pathway
    Article Snippet: RNA was extracted from the HUVECs using Trizol (Ambion; Thermo Fisher Scientific, Inc.). .. Primarily, RNA was reverse transcribed into cDNA using HiScript Reverse Transcriptase (Vazyme Biotech Co., Ltd.) according to the manufacturer's protocol. .. The mRNA expression level was then determined using SYBR qPCR Master Mix (Vazyme Biotech Co., Ltd.) alongside specific primers ( ) on an eQ9600 PCR System (Eastwin Life Sciences; Beijing Dongsheng Innovation Biotechnology Co., Ltd.).

    Article Title: Dexmedetomidine suppresses ferritinophagy-mediated ferroptosis in myocardial ischemia/reperfusion injury via the YAP1/NCOA4 pathway.
    Article Snippet: The present study aimed to investigate the mechanism by which dexmedetomidine (Dex) mitigates myocardial ischemia/reperfusion injury (MIRI) through the regulation of ferritinophagy-mediated ferroptosis.. In vivo, a rat MIRI model was established by surgical ligation of the coronary artery to evaluate the infarct area and histopathological changes.. In vitro, H9c2 cardiomyocytes were subjected to hypoxia/reoxygenation (H/R) treatment to assess cell viability, lipid peroxidation levels, intracellular Fe2+ levels, and mitochondrial and autophagosome morphology using transmission electron microscopy (TEM).

    Article Title: Changyanning Ameliorates Dextran Sodium Sulfate-Induced Colitis by Modulating Gut Microbiota Inflammation and Intestinal Barrier in Mice.
    Article Snippet: The rising prevalence of colitis and the limitations of existing therapies necessitate novel therapeutic agents.. Unlike studies focusing on single compounds, this research elucidates the integrated therapeutic mechanism of the traditional Chinese medicine formula Changyanning (CYN), specifically targeting the synergistic regulatory effects on inflammatory responses, intestinal barrier function, and gut microbiota during dextran sodium sulfate (DSS)-induced colitis.. In a male C57BL/6 mouse model, CYN treatment significantly ameliorated clinical symptoms and histopathological damage.

    Article Title: Trophic transfer of chromium from Bellamya aeruginosa drives edema in medicinal leech Whitmania pigra via the ROS-FoxO-ENaC signaling.
    Article Snippet: • Bellamya aeruginosa bioaccumulates Cr (VI) and transmits it through the food chain causing Whitmania pigra edema.. • Cr (VI) exposure distrupts gut microbiota, elevated ROS, and activates FoxO signaling in W. pigra.. • Cr (VI)-caused redox imbalance altered expression of ENaCα and Na+/K+ATPase, causing fluid retention.

    Article Title: Combinatorial inhibition of IGF2BP3-regulated receptor tyrosine kinases offers a new therapeutic strategy for triple-negative breast cancer.
    Article Snippet: RNAprotein complexes were immunoprecipitated using Protein A/G magnetic beads (MedChemExpress, New Jersey, USA) for 2 h at 4°C. .. Following extensive washing with RIP wash buffer, bound RNAs were eluted, purified using the RNeasy Mini Kit (Qiagen, Hilden, Germany), reverse-transcribed into cDNA with HiScript® Reverse Transcriptase (Vazyme, Nanjing, China), and quantified by qRT-PCR. .. For MeRIP, total RNA was extracted using TRIzolTM reagent (Takara,

    Purification:

    Article Title: Combinatorial inhibition of IGF2BP3-regulated receptor tyrosine kinases offers a new therapeutic strategy for triple-negative breast cancer
    Article Snippet: RNA-protein complexes were immunoprecipitated using Protein A/G magnetic beads (MedChemExpress, New Jersey, USA) for 2 h at 4 °C. .. Following extensive washing with RIP wash buffer, bound RNAs were eluted, purified using the RNeasy Mini Kit (Qiagen, Hilden, Germany), reverse-transcribed into cDNA with HiScript ® Reverse Transcriptase (Vazyme, Nanjing, China), and quantified by qRT-PCR. .. For MeRIP, total RNA was extracted using TRIzolTM reagent (Takara, Kyoto, Japan).

    Article Title: Combinatorial inhibition of IGF2BP3-regulated receptor tyrosine kinases offers a new therapeutic strategy for triple-negative breast cancer.
    Article Snippet: RNAprotein complexes were immunoprecipitated using Protein A/G magnetic beads (MedChemExpress, New Jersey, USA) for 2 h at 4°C. .. Following extensive washing with RIP wash buffer, bound RNAs were eluted, purified using the RNeasy Mini Kit (Qiagen, Hilden, Germany), reverse-transcribed into cDNA with HiScript® Reverse Transcriptase (Vazyme, Nanjing, China), and quantified by qRT-PCR. .. For MeRIP, total RNA was extracted using TRIzolTM reagent (Takara,

    cDNA Synthesis:

    Article Title: Trophic transfer of chromium from Bellamya aeruginosa drives edema in medicinal leech Whitmania pigra via the ROS-FoxO-ENaC signaling.
    Article Snippet: • Bellamya aeruginosa bioaccumulates Cr (VI) and transmits it through the food chain causing Whitmania pigra edema.. • Cr (VI) exposure distrupts gut microbiota, elevated ROS, and activates FoxO signaling in W. pigra.. • Cr (VI)-caused redox imbalance altered expression of ENaCα and Na+/K+ATPase, causing fluid retention.



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